ABSTRACT
The present work is to study the chemical constituents from petroleum ether fraction of Tibetan medicine Swertia chirayita by column chromatography and recrystallization. The structures were identified by physical and chemical properties and spectral data as swerchirin (1), decussatin (2), 1,8-dihydroxy-3,5,7-trimethoxyxanthone (3), 1-hydroxy-3,5,7,8-tetramethoxyxanthone (4), bellidifolin (5), 1-hydroxy-3, 7-dimethoxyxanthone (6), methylswertianin (7), 1-hydroxy-3,5-dimethoxyxanthone (8), erythrodiol (9), oleanolic acid (10), gnetiolactone (11), scopoletin (12), sinapaldehyde (13), syringaldehyde (14), and β-sitosterol (15). Compounds 3, 4, 9, 11-14 were isolated from S. chirayita for the first time. Compounds 9 and 12 were firstly isolated from the genus Swertia. The cytotoxic activities of compounds 1, 2, 5, 7 and 8 against human pancreatic cancer cell lines SW1990 and BxPC-3,and the protective effects of these compounds against hydrogen peroxide (H2O2)-induced oxidative stress in human endothelium-derived EA.hy926 were investigated in vitro. The results showed no obvious effect at the high concentration of 50 μmol•L⁻¹.
ABSTRACT
To study the chemical constituents of the fruits of Polygonum orientale L., silica gel and ODS column chromatography methods were used to separate and purify the constituents. Their structures were elucidated on the basis of physicochemical properties and spectral analysis. Three compounds were identified as 28-O-beta-D-glucopyranosyl-3beta, 7beta-dihydroxy-lup-20 (29)-en-28-oate (1), 5,7-dihydroxychromone (2) and naringenin (3). Compound 1 is a new triterpenoid saponin and others were isolated from the fruits of this plant for the first time.
Subject(s)
Chromones , Chemistry , Flavanones , Chemistry , Fruit , Chemistry , Molecular Structure , Plants, Medicinal , Chemistry , Polygonum , Chemistry , Saponins , Chemistry , Triterpenes , ChemistryABSTRACT
<p><b>OBJECTIVE</b>To study the chemical constituents of the aerial parts of Liusticum chuanxiong.</p><p><b>METHOD</b>The chemical components were isolated by silica gel and Sephadex LH-20 column chromatography. Structures were elucidated on the basis of physico-chemical properities and spectral data.</p><p><b>RESULT</b>Eight chemical constituents were isolated, and identified as protocatechuic acid (1), caffeic acid (2), scopoletin (3), apigenin (4), quercetin (5), cosmosiin (6), kaempferol-3-O-beta-D-glucopyranosid (7) and glucose (8).</p><p><b>CONCLUSION</b>Compounds 1-8 were obtained from the aerial parts of the plant for the first time, compounds 3-8 were obtained from the plant for the first time.</p>
Subject(s)
Apigenin , Chemistry , Kaempferols , Chemistry , Ligusticum , Chemistry , Plant Components, Aerial , Chemistry , Plants, Medicinal , Chemistry , Scopoletin , ChemistryABSTRACT
<p><b>OBJECTIVE</b>To study the chemical constituents of Prunella vulgaris.</p><p><b>METHOD</b>To separate the constituents of P. vulgaris by using various kinds of chromatography and identify their structures on the basis of spectral analysis.</p><p><b>RESULT</b>Seven compounds were isolated from the spikes of P. vulgaris. Their structures were established as autantiamide acetate (1), rhein (2), tanshinone I (3), danshensu (4), stigmast-7, 22-dien-3-one (5), 3, 4, alpha-trihydroxy-methyl phenylpropionate (6), butyl rosmarinate (7).</p><p><b>CONCLUSION</b>Compounds 1-4 were isolated from this genus for the first time.</p>
Subject(s)
Amides , Chemistry , Anthraquinones , Chemistry , Chromatography, High Pressure Liquid , Abietanes , Flowers , Chemistry , Lactates , Chemistry , Magnetic Resonance Spectroscopy , Phenanthrenes , Chemistry , Plants, Medicinal , Chemistry , Prunella , ChemistryABSTRACT
<p><b>OBJECTIVE</b>To promote its comprehensive utilization, the involatile constituents of Mentha haplocalyx were studied systematically.</p><p><b>METHOD</b>The chemical components were isolated and purified by silca gel column chromatography and recrystallization. The chemical structures were elucidated on the basis of physico-chemical properties and spectral data.</p><p><b>RESULT</b>Eight compounds were isolated and identified as: acacetin (I), tilianine (II), linarin (III), n-butyl-beta-D-fructopyranoside (IV), ursolic acid (V), oleanolic acid (VI), beta-sitosterol (VII), daucosterol (VIII).</p><p><b>CONCLUSION</b>Compounds I approximately V were obtained from this plant for the first time.</p>
Subject(s)
Flavones , Chemistry , Glycosides , Chemistry , Mentha , Chemistry , Plant Components, Aerial , Chemistry , Plants, Medicinal , Chemistry , Triterpenes , ChemistryABSTRACT
<p><b>AIM</b>To study the chemical constituents of Glechoma longituba (Nakai) Kupr.</p><p><b>METHODS</b>Manifold chromatography methods were used to separated the chemical constituents, and the chemical structures were determined by spectral analyses.</p><p><b>RESULTS</b>Nine compounds were isolated from Glechoma longituba and identified, as: glecholone (1), 6R,9R-3-oxo-alpha-ionol (2), S(+)-dehydrovomifoliol (3), vomifoliol (4), corosolic acid (5), quercetin (6), stigmastenol (7), myristic acid (8) and triacontanol (9).</p><p><b>CONCLUSION</b>Compound 1 is a new compound. Compounds 2 - 9 were isolated from this plant for the first time.</p>
Subject(s)
Cyclohexanones , Chemistry , Lamiaceae , Chemistry , Molecular Conformation , Molecular Structure , Plant Components, Aerial , Chemistry , Plants, Medicinal , Chemistry , Triterpenes , ChemistryABSTRACT
<p><b>OBJECTIVE</b>Study on the flavonoids from the air part of Eupatorium lindleyanum.</p><p><b>METHOD</b>Alcohol percolation, silica gel column chromatography and re-crystallization et al.</p><p><b>RESULT</b>Jaceosidin, kaempferol, quceritin, astragalin, trifolin and hypersoide were isolated from E. lindleyanum. Their structures were elucidated on the basis of spectral evidence.</p><p><b>CONCLUSION</b>Jaceosidin, kaempferol, quceritin, astragalin and trifolin were isolated from E. lindleyanum for the first time.</p>
Subject(s)
Eupatorium , Chemistry , Flavonoids , Chemistry , Kaempferols , Chemistry , Plant Components, Aerial , Chemistry , Plants, Medicinal , Chemistry , Quercetin , ChemistryABSTRACT
<p><b>OBJECTIVE</b>To study the anticancer activity of the extract from Citrus reticulata in vivo.</p><p><b>METHOD</b>Anticancer activities were tested with tumor model in vivo (Sarcoma-180 cells, Heps cells, EAC cells implanted in mice).</p><p><b>RESULT</b>The extract from Citrus reticulata showed marked anticancer activities on Sarcoma-180 cells and Heps cells implanted in mice, had no marked anticancer activities on EAC cells implanted in mice and induced apoptosis of Sarcoma-180 cell.</p><p><b>CONCLUSION</b>The extract from Citrus reticulata will have promising prospects as an anticancer Chinese medicine, but further studies will be needed.</p>